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primerscript rt reagent kit  (TaKaRa)


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    TaKaRa primerscript rt reagent kit
    Primerscript Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 87884 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primerscript+rt+reagent+kit/PrimeScript+RT+Reagent+Kit/pm42028593-72-9-14
    Average 99 stars, based on 87884 article reviews
    primerscript rt reagent kit - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Real-time Polymerase Chain Reaction:

    Article Title: Lnc1267-hnRNP U interaction promotes radioresistance by inhibiting apoptosis via attenuated RelA/p65 Ser536 phosphorylation.
    Article Snippet: .. For qPCR, beads were treated with protease K buffer, RNA was extracted using phenol-chloroform, and reverse transcribed using PrimerScript RT reagent Kit (Takara, RR047A, Japan). qPCR was performed with 2× M5 Hiper SYBR Premix EsTaq kit (Mei5 Biotechnology, MF787-02, China). ..

    Article Title: POSTN + fibroblast-secreted small extracellular vesicles drive macrophage M2 polarization through BMP4/BMPR2/Smad signaling
    Article Snippet: RNA concentration was quantified using a Nanodrop One spectrophotometer (Thermo Fisher Scientific, Inc.). .. Reverse transcription was performed with the PrimerScript RT reagent Kit (Takara Biotechnology Co., Ltd.) according to the manufacture's protocol, followed by quantitative real-time PCR using the SYBR Premix Ex Taq reagent kit (Takara Biotechnology Co., Ltd.) according to the manufacture's protocol on a LightCycler 480II system (Roche Diagnostics) with the following cycling protocol: Initial denaturation at 95°C for 15 min; followed by 40 cycles of denaturation at 95°C for 10 sec, annealing at 56–60°C for 20 sec and extension at 72°C for 32 sec. GAPDH was used as an internal control. ..

    Article Title: POSTN + fibroblast‑secreted small extracellular vesicles drive macrophage M2 polarization through BMP4/BMPR2/Smad signaling.
    Article Snippet: RNA concentration was quantified using a Nanodrop One spectrophotometer (Thermo Fisher Scientific, Inc.). .. Reverse transcription was performed with the PrimerScript RT reagent Kit (Takara Biotechnology Co., Ltd.) according to the manufacture's protocol, followed by quantitative real‐time PCR using the SYBR Premix Ex Taq reagent kit (Takara Biotechnology Co., Ltd.) according to the manufacture's protocol on a LightCycler 480II system (Roche Diagnostics) with the following cycling protocol: Initial denaturation at 95 ̊C for 15 min; followed by 40 cycles of dena‐ turation at 95 ̊C for 10 sec, annealing at 56‐60 ̊C for 20 sec and extension at 72 ̊C for 32 sec. GAPDH was used as an internal control. ..

    Reverse Transcription:

    Article Title: Lnc1267-hnRNP U interaction promotes radioresistance by inhibiting apoptosis via attenuated RelA/p65 Ser536 phosphorylation.
    Article Snippet: .. For qPCR, beads were treated with protease K buffer, RNA was extracted using phenol-chloroform, and reverse transcribed using PrimerScript RT reagent Kit (Takara, RR047A, Japan). qPCR was performed with 2× M5 Hiper SYBR Premix EsTaq kit (Mei5 Biotechnology, MF787-02, China). ..

    Article Title: POSTN + fibroblast-secreted small extracellular vesicles drive macrophage M2 polarization through BMP4/BMPR2/Smad signaling
    Article Snippet: RNA concentration was quantified using a Nanodrop One spectrophotometer (Thermo Fisher Scientific, Inc.). .. Reverse transcription was performed with the PrimerScript RT reagent Kit (Takara Biotechnology Co., Ltd.) according to the manufacture's protocol, followed by quantitative real-time PCR using the SYBR Premix Ex Taq reagent kit (Takara Biotechnology Co., Ltd.) according to the manufacture's protocol on a LightCycler 480II system (Roche Diagnostics) with the following cycling protocol: Initial denaturation at 95°C for 15 min; followed by 40 cycles of denaturation at 95°C for 10 sec, annealing at 56–60°C for 20 sec and extension at 72°C for 32 sec. GAPDH was used as an internal control. ..

    Article Title: POSTN + fibroblast‑secreted small extracellular vesicles drive macrophage M2 polarization through BMP4/BMPR2/Smad signaling.
    Article Snippet: RNA concentration was quantified using a Nanodrop One spectrophotometer (Thermo Fisher Scientific, Inc.). .. Reverse transcription was performed with the PrimerScript RT reagent Kit (Takara Biotechnology Co., Ltd.) according to the manufacture's protocol, followed by quantitative real‐time PCR using the SYBR Premix Ex Taq reagent kit (Takara Biotechnology Co., Ltd.) according to the manufacture's protocol on a LightCycler 480II system (Roche Diagnostics) with the following cycling protocol: Initial denaturation at 95 ̊C for 15 min; followed by 40 cycles of dena‐ turation at 95 ̊C for 10 sec, annealing at 56‐60 ̊C for 20 sec and extension at 72 ̊C for 32 sec. GAPDH was used as an internal control. ..

    Article Title: Prunetin Protects Against Sepsis-Induced Acute Lung Injury by Inhibiting Inflammation and Oxidative Stress via Regulating the TLR4/NF-κB/NLRP3 Inflammasome Pathway.
    Article Snippet: Prunetin is a naturally occurring flavonoid with effective therapeutic potential against diverse diseases.. This study aimed to investigate the potential properties of prunetin in alleviating lipopolysaccharide (LPS)induced inflammatory responses and oxidative stress in an acute lung injury (ALI) mouse model and murine alveolar macrophages (MHS cells).. The results showed that prunetin protected against LPSinduced ALI in mice, as shown by improved histopathological alterations in lung tissues, reduced lung injury score, and decreased lung wet/dry weight ratio.

    Synthesized:

    Article Title: The genome-wide identification and gonad-specific expression analysis of the PfTSSK gene family in Pinctada fucata.
    Article Snippet: Pinctada fucata is a crucial marine pearl oyster species for pearl culture, and the regulation of gonadal maturation is essential for pearl oyster breeding.. This study investigates the genetic characteristics and expression patterns of the testis-specific serine/threonine kinase family (PfTSSK) in this species.. A total of six PfTSSK genes were identified, namely PfTSSK1, PfTSSK1-like, PfTSSK3, PfTSSK4, PfTSSK5, and PfTSSK7, among which PfTSSK1-like is a newly reported member in P. fucata.

    Isolation:

    Article Title: Identification and functional characterization of heat shock transcription factor genes in Rhododendron Delavayi under heat stress.
    Article Snippet: .. The isolated mRNA was converted single- and double-stranded cDNA using the PrimerScript RT Reagent Kit (TaKaRa, Dalian, China). ..

    Article Title: Identification and functional characterization of heat shock transcription factor genes in Rhododendron Delavayi under heat stress
    Article Snippet: .. The isolated mRNA was converted single- and double-stranded cDNA using the PrimerScript RT Reagent Kit (TaKaRa, Dalian, China). ..

    Control:

    Article Title: POSTN + fibroblast-secreted small extracellular vesicles drive macrophage M2 polarization through BMP4/BMPR2/Smad signaling
    Article Snippet: RNA concentration was quantified using a Nanodrop One spectrophotometer (Thermo Fisher Scientific, Inc.). .. Reverse transcription was performed with the PrimerScript RT reagent Kit (Takara Biotechnology Co., Ltd.) according to the manufacture's protocol, followed by quantitative real-time PCR using the SYBR Premix Ex Taq reagent kit (Takara Biotechnology Co., Ltd.) according to the manufacture's protocol on a LightCycler 480II system (Roche Diagnostics) with the following cycling protocol: Initial denaturation at 95°C for 15 min; followed by 40 cycles of denaturation at 95°C for 10 sec, annealing at 56–60°C for 20 sec and extension at 72°C for 32 sec. GAPDH was used as an internal control. ..

    Article Title: POSTN + fibroblast‑secreted small extracellular vesicles drive macrophage M2 polarization through BMP4/BMPR2/Smad signaling.
    Article Snippet: RNA concentration was quantified using a Nanodrop One spectrophotometer (Thermo Fisher Scientific, Inc.). .. Reverse transcription was performed with the PrimerScript RT reagent Kit (Takara Biotechnology Co., Ltd.) according to the manufacture's protocol, followed by quantitative real‐time PCR using the SYBR Premix Ex Taq reagent kit (Takara Biotechnology Co., Ltd.) according to the manufacture's protocol on a LightCycler 480II system (Roche Diagnostics) with the following cycling protocol: Initial denaturation at 95 ̊C for 15 min; followed by 40 cycles of dena‐ turation at 95 ̊C for 10 sec, annealing at 56‐60 ̊C for 20 sec and extension at 72 ̊C for 32 sec. GAPDH was used as an internal control. ..



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    primerscript rt reagent kit  (TaKaRa)
    99
    TaKaRa primerscript rt reagent kit
    Primerscript Rt Reagent Kit, supplied by TaKaRa, used in various techniques. Bioz Stars score: 99/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/primerscript+rt+reagent+kit/PrimeScript+RT+Reagent+Kit/pm42028593-72-9-14
    Average 99 stars, based on 1 article reviews
    primerscript rt reagent kit - by Bioz Stars, 2026-09
    99/100 stars
    		  Buy from Supplier

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